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93328

Sigma-Aldrich

Trizma® maleate

BioUltra, ≥99.5% (NT)

Synonym(s):

TRIS maleate salt, Tris(hydroxymethyl)aminomethane maleate salt, Tromethamine maleate salt

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About This Item

Linear Formula:
NH2C(CH2OH)3 · C4H4O4
CAS Number:
72200-76-1
Molecular Weight:
237.21
EC Number:
276-455-6
MDL number:
MFCD00082442
UNSPSC Code:
12161700
PubChem Substance ID:
57653534
NACRES:
NA.25

product line

BioUltra

Quality Level

100

Assay

≥99.5% (NT)

form

powder or crystals

impurities

insoluble matter, passes filter test

ign. residue (900 °C)

≤0.1% (as SO4)

pH

3.0-4.5 (25 °C, 0.5 M in H2O)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

absorption

cut-off at 328 nm in H2O at 0.5 M

SMILES string

NC(CO)(CO)CO.OC(=O)\C=C/C(O)=O

InChI

1S/C4H11NO3.C4H4O4/c5-4(1-6,2-7)3-8;5-3(6)1-2-4(7)8/h6-8H,1-3,5H2;1-2H,(H,5,6)(H,7,8)/b;2-1-

InChI key

HTMWOUBCEZXSHN-BTJKTKAUSA-N

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Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma maleate is used in the preparation of Tris maleate buffers with lower effective pH buffering capacity than Tris buffers. Tris maleate buffers may be used in various grow media, in tissue preparations and as a diluent or washing buffer.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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