B9431

Biotin-4-Fluorescein

≥95% purity (HPLC), powder

• Synonym(s): 5-(6)-[2-(biotinoyl)aminoethylaminocarbonyl] fluorescein, B4F, Biotin (5-fluorescein) conjugate
• Empirical Formula (Hill Notation): C₃₃H₃₂N₄O₈S
• Molecular Weight: 644.69
• UNSPSC Code: 12171500
• eCl@ss: 34058011
• PubChem Substance ID: 329773319
• NACRES: NA.47

Properties

• product name: Biotin-4-Fluorescein, ≥95.0% (HPLC)
• Quality Level: 100
• Assay: ≥95.0% (HPLC)
• form: powder
• mol wt: 644.71
• technique(s): titration: suitable
• color: light orange to very dark orange; light yellow to very dark yellow
• ε (extinction coefficient): ≥70 at 491-497 nm in phosphate buffer at 0.05 M
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56

Description

Application

Biotin-4-Fluorescein has been used for the labeling of avidin to study the changes in protein binding capability after carbon negative-ion implantation into avidin. It has been used to study the binding affinity of streptavidin towards biotin.

Useful for quantitating biotin binding sites. This reagent binds to avidin more quickly than conventional fluorescein biotin.

Biochem/physiol Actions

Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenching, which is used for measuring the concentration of (strept)avidin.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves