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222550

Sigma-Aldrich

Nile Blue chloride

Dye content 85 %

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About This Item

Empirical Formula (Hill Notation):
C20H20ClN3O
CAS Number:
2381-85-3
Molecular Weight:
353.85
Colour Index Number:
51180
Beilstein:
3923952
MDL number:
MFCD00011936
UNSPSC Code:
12171500
PubChem Substance ID:
24853342
NACRES:
NA.47

form

powder

Quality Level

200

composition

Dye content, 85%

solubility

water: 1 mg/mL, opaque, blue to very deep blue

λmax

638 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34

InChI

1S/C20H19N3O.ClH/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;/h5-12,21H,3-4H2,1-2H3;1H

InChI key

SHXOKQKTZJXHHR-UHFFFAOYSA-N

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Application

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.

Biochem/physiol Actions

Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0). Nile blue chloride has been used to detect DNA and lipids. In addition, it also helps to identify bacterial growth. Nile blue chloride also facilitates diagnosis of disease linked with amyloid accumulation.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBQ1689
SHBN8805
SHBM3768
11802LZ
06503TR

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R. W. Sabnis
Handbook of Acid-Base Indicators (2007)
Zhiping Cui et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 83(1), 1-7 (2011-09-29)
A dual-wavelength overlapping resonance Rayleigh scattering (DWO-RRS) method was developed to detect chondroitin sulfate (CS) with nile blue sulfate (NBS). At pH 3.0-4.0 Britton-Robinson (BR) buffer medium, CS interacted with NBS to form an ion-association complex. As a result, the
Julie Dutet et al.
Pharmaceutical research, 29(12), 3464-3474 (2012-08-08)
To estimate the in vitro ungual penetration depth of sodium fluorescein and nile blue chloride by laser scanning confocal microscopy. The depth, uniformity and pathways of penetration of both markers into human nail during passive and iontophoretic experiments were investigated.
Cai Wang et al.
Journal of economic entomology, 105(5), 1758-1765 (2012-11-20)
The consumption and food transfer efficiency of two commercially used termite bait materials, southern yellow pine wood and cardboard, and one potential bait material, maize (Zea mays L.) cob, were evaluated for use against the Formosan subterranean termite, Coptotermes formosanus
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