Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

11633716001

Roche

Anti-Digoxigenin-POD (poly), Fab fragments

from sheep

Synonym(s):

anti-digoxigenin, digoxigenin

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203

biological source

sheep

Quality Level

100

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

packaging

pkg of 50 U

manufacturer/tradename

Roche

isotype

IgG

storage temp.

2-8°C

General description

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to polymerized horseradish peroxidase.

Specificity

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Application

Anti-Digoxigenin-POD (poly), Fab fragments has been used for the detection of digoxigenin-labeled compounds using:
  • whole-mount in situ hybridization
  • fluorescence in situ hybridization
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • Southern blot
  • western blot
  • double labeling experiments
  • lectin blots

Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.
Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
  • Dot blot: 50 to 100 mU/ml
  • ELISA: 2 to 50 mU/ml
  • Western blot: 50 to 100 mU/ml
  • Southern blot: 50 to 100 mU/ml

Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Reconstitution

Add 1 ml double-distilled water to a final concentration of 50 U/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Not finding the right product?  

Try our Product Selector Tool.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Potent and selective BACE-1 peptide inhibitors lower brain Abeta levels mediated by brain shuttle transport
<BIG>Ruderisch N, et al.</BIG>
EBioMedicine, 24, 76-92 (2017)
Christopher R Campos et al.
PloS one, 15(3), e0229850-e0229850 (2020-03-11)
Reducing Amyloid β (Aβ) in the brain is of fundamental importance for advancing the therapeutics for Alzheimer`s disease. The endogenous metallopeptidase neprilysin (NEP) has been identified as one of the key Aβ-degrading enzymes. Delivery of NEP to the brain by
Louise Heydenreich Jensen et al.
International archives of allergy and immunology, 178(4), 307-314 (2019-02-14)
Infant formulas (IFs) based on hydrolysed cow's milk proteins are central in the management of cow's milk allergy (CMA) in infants and small children. New IF compositions with improved prevention and treatment properties are needed, along with appropriate preclinical animal
In Situ Hybridization Methods (2015)
Maya K Leabman et al.
mAbs, 5(6), 896-903 (2014-02-05)
Antibody interactions with Fcγ receptors (FcγRs), like FcγRIIIA, play a critical role in mediating antibody effector functions and thereby contribute significantly to the biologic and therapeutic activity of antibodies. Over the past decade, considerable work has been directed towards production
View All Related Papers

Articles

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service