MAB5272
Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324
clone 324, Chemicon®, from rat
Synonym(s):
CD171, N-CAM L1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
324, monoclonal
species reactivity
mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... L1CAM(3897) , VCAM1(7412)
General description
Families of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules: N-CAM, myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts
Specificity
Monoclonal antibody against neural cell adhesion molecule L1 from rat-rat hybrid cells.
Immunogen
Rats were immunized with a glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice.
Application
This Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 is validated for use in IC, IH, WB for the detection of Neural Cell Adhesion Molecule L1.
Western blotting:
20 μg/mL was used on a previous lot.
Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.
Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.
Optimal working dilutions must be determined by end user.
20 μg/mL was used on a previous lot.
Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.
Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.
Optimal working dilutions must be determined by end user.
Target description
120-140 kDa
Physical form
Format: Purified
Rat monoclonal in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Neuron glia-related cell adhesion molecule (NrCAM) promotes topographic retinocollicular mapping.
Dai, J; Buhusi, M; Demyanenko, GP; Brennaman, LH; Hruska, M; Dalva, MB; Maness, PF
Testing null
ADAM17 is critical for multipolar exit and radial migration of neuronal intermediate progenitor cells in mice cerebral cortex.
Li, Q; Zhang, Z; Li, Z; Zhou, M; Liu, B; Pan, L; Ma, Z; Zheng, Y
Testing null
Evidence that descending cortical axons are essential for thalamocortical axons to cross the pallial-subpallial boundary in the embryonic forebrain.
Chen, Y; Magnani, D; Theil, T; Pratt, T; Price, DJ
Testing null
Yuhki Saito et al.
eLife, 5 (2016-05-26)
The neuron specific RNA-binding proteins NOVA1 and NOVA2 are highly homologous alternative splicing regulators. NOVA proteins regulate at least 700 alternative splicing events in vivo, yet relatively little is known about the biologic consequences of NOVA action and in particular
C J Bott et al.
Molecular biology of the cell, 30(10), 1214-1229 (2019-03-07)
Correct wiring in the neocortex requires that responses to an individual guidance cue vary among neurons in the same location, and within the same neuron over time. Nestin is an atypical intermediate filament expressed strongly in neural progenitors and is
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