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G005899

HPLC Analysis of a Monoclonal Antibody on Zenix® SEC-300 after Partial Proteolysis with IdeS Protease

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Zenix SEC-300, 30 cm X 4.6 mm I.D., 3 μm particles (Z777087)
mobile phase: 0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide
flow rate: 0.35 mL/min
pressure: 1350 psi (93 bar)
column temp.: ambient
detector: UV, 215 nm
injection: 1 μL
sample: digested SO57 mAb, ~0.5 g/L

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

Proteolysis of antibodies can be useful for simplifying further analysis. This application demonstrates separation of the two fragments, F(ab′)2 and scFc from the intact IgG, after partial digestion with IdeS protease.

Legal Information

Zenix is a registered trademark of Sepax Technologies

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