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G005736

LC/MS Analysis of Sertraline and Norsertraline on Ascentis® Express RP-Amide

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP Amide, 10 cm x 3.0 mm I.D., 2.7 μm particles (53918-U)
mobile phase: [A] 10 mM ammonium formate, water, pH 3.6; [B] 10 mM ammonium formate, acetonitrile, pH 3.6; (65:35, A:B)
flow rate: 0.6 mL/min
column temp.: 55 °C
detector: ESI(+), 100-1000 m/z
injection: 2 μL
sample: 200 ng/mL in 25:75, water: acetonitrile

suitability

application for HPLC

application(s)

clinical
pharmaceutical (small molecule)

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

mobile phase component


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