ZRB1173
Anti-phospho JNK (Thr183, Tyr185) Antibody, clone 3F7, ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3F7, recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 48.29 kDa
species reactivity
human, mouse
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable (peptide)
isotype
IgG
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
phosphorylation (pThr183/pTyr185)
Gene Information
human ... MAPK8(5599)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 3F7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects JNK1/2 phosphorylated on threonine 183 and tyrosine 185.
Immunogen
KLH-conjugated linear peptide corresponding to 10 amino acids surrounding phosphothreonine 183 and phosphotyrosine 185 from human JNK/SAPK.
Application
Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb , Cat. No. ZRB1173, is a highly specific recombinant rabbit monoclonal antibody that detects JNK/SAPK phosphorylated on Thr183 and Tyr185 and has been tested for use in Immunocytochemistry, Immunohistochemistry, Peptide Inhibition Assay, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho JNK (Thr183, Tyr185) in NIH3T3 cells treated with Anisomyocin.
Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected phospho JNK (Thr183, Tyr185) in human colon cancer and human ovarian cancer tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho JNK (Thr183, Tyr185) in HeLa cells treated with Anisomycin treatment.
Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot was used with NIH3T3 cells treated with Anisomyocin ((25 ug/ml for 30 min following overnight serum starvation) for peptide block analysis.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected phospho JNK (Thr183, Tyr185) in human colon cancer and human ovarian cancer tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho JNK (Thr183, Tyr185) in HeLa cells treated with Anisomycin treatment.
Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot was used with NIH3T3 cells treated with Anisomyocin ((25 ug/ml for 30 min following overnight serum starvation) for peptide block analysis.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
Target description
Mitogen-activated protein kinase 8/9 (UniProt: P45983/P45984; also known as MAP kinase 8/9, MAPK8/9, Stress-activated protein kinase 1c/1a, SAPK1c/SAPK1a, Stress-activated protein kinase JNK1/ JNK2, c-Jun N-terminal kinase 1/2) is encoded by the MAPK8/9 (also known as JNK1/JNK2, PRKM8/9, SAPK1/2, SAPK1C/1A) gene (Gene ID: 5599/ 5601) in human. SAPK/JNK is a serine/threonine-protein kinase that is involved in various biological processes, including cell proliferation, differentiation, migration, transformation, and programmed cell death. It is activated in response to extracellular stimuli such as proinflammatory cytokines or physical stress. Both JNK1 and JNK2 contain a TXY motif (aa 183-185) that contains the threonine and tyrosine residues whose phosphorylation activates these kinases. Protein kinase domain of SAPK/JNK is localized to amino acids 26-321. Phosphorylation and activation of SAPK/JNK is achieved by two dual specificity kinases, MAP2K7 and MAP2K4. Following their activation SAPK/JNK phosphorylate several transcription factors, primarily components of AP-1 such as JUN, JDP2, and ATF2 and thus regulates AP-1 transcriptional activity. It promotes apoptotic cell death in stressed cells by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. The immunogen sequence used to generate this clone is conserved in both JNK1 and JNK2. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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