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Y2377

Sigma-Aldrich

2x YT medium

Powder microbial growth medium

Synonym(s):

2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media

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About This Item

UNSPSC Code:
41106225
NACRES:
NA.85

grade

for molecular biology

sterility

non-sterile

form

powder

technique(s)

microbiological culture: suitable

pH

6.8-7.2(3.1% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

General description

2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

2x YT medium has been used for culturing E. coli BL21 for cloning , E. coli SS320 cells for scaffold synthesis , and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.

Features and Benefits

2xYT powder provides:
  • Standard formulation
  • An inexpensive alternative to liquid

Components

16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl

Preparation Note

1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.

Reconstitution

Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Designed active-site library reveals thousands of functional GFP variants
Weinstein J Y. et al.
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Telomere-mediated Chromosomal Truncation via Agrobacterium tumefaciens or Particle Bombardment to Produce Engineered Minichromosomes in Plants
Graham ND, et al.
Bio-protocol, 5(18) (2015)
Bacterial artificial chromosomes: A functional genomics tool for the study of positive-strand RNA viruses
Yun SI, et al.
Journal of Visualized Experiments, 106, e53164-e53164 (2015)
Rebecca R Du et al.
ACS nano, 16(12), 20340-20352 (2022-12-03)
Three-dimensional wireframe DNA origami have programmable structural and sequence features that render them potentially suitable for prophylactic and therapeutic applications. However, their innate immunological properties, which stem from parameters including geometric shape and cytosine-phosphate-guanine dinucleotide (CpG) content, remain largely unknown.
Basem Al-Shayeb et al.
Cell, 185(24), 4574-4586 (2022-11-25)
CRISPR-Cas systems are host-encoded pathways that protect microbes from viral infection using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find that CRISPR systems are also encoded in diverse bacteriophages, where they occur as divergent and hypercompact anti-viral systems. Bacteriophage-encoded

Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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