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WTA1
TransPlex® Whole Transcriptome Amplification Kit
DNA polymerase separate.
Synonym(s):
Transcriptome Amplification Kit
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About This Item
UNSPSC Code:
41121800
NACRES:
NA.55
€3,250.00
Please contact Customer Service for Availability
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General description
TransPlex®, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.
Application
TransPlex® Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA.[1] It has also been used in the amplification of cDNA.[2]
Suitable for use with downstream applications including:
- qPCR
- microarray analysis
- cloning
Features and Benefits
- Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time
- Only 5 ng of starting material required to produce a highly representative library from total RNA
- Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples
Principle
The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.
Legal Information
TransPlex is a registered trademark of Rubicon Genomics, Inc.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Sa Xiao et al.
Virus research, 145(1), 80-91 (2009-06-23)
The complete genome sequence of avian paramyxovirus serotype 7 (APMV-7) prototype strain dove/Tennessee/4/75 was determined. The genome size is 15,480 nucleotides (nt) long and follows the "rule of six". The genome contains six non-overlapping genes in the order of 3'-N-P/V/W-M-F-HN-L-5'.
Munehiro Okamoto et al.
Scientific reports, 5, 8850-8850 (2015-03-07)
We discovered a lethal hemorrhagic syndrome arising from severe thrombocytopenia in Japanese macaques kept at the Primate Research Institute, Kyoto University. Extensive investigation identified that simian retrovirus type 4 (SRV-4) was the causative agent of the disease. SRV-4 had previously
Catarina Guimarães-Teixeira et al.
Journal of personalized medicine, 11(10) (2021-10-24)
(1) Background: Methylation of N6-adenosine (m6A) is the most abundant messenger RNA (mRNA) modification in eukaryotes. We assessed the expression profiles of m6A regulatory proteins in renal cell carcinoma (RCC) and their clinical relevance, namely, as potential biomarkers. (2) Methods:
Complete genome sequence of avian paramyxovirus type 7 (strain Tennessee) and comparison with other paramyxoviruses
Xiao S, et al.
Virus Research, 145(1), 80-91 (2009)
Ki Wook Kim et al.
Open forum infectious diseases, 6(2), ofz025-ofz025 (2019-03-01)
The importance of gut bacteria in human physiology, immune regulation, and disease pathogenesis is well established. In contrast, the composition and dynamics of the gut virome are largely unknown; particularly lacking are studies in pregnancy. We used comprehensive virome capture
Protocols
TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.
TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.
TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.
TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.
Related Content
Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications
Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications
Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications
Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications
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What is the Department of Transportation shipping information for this product?
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Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used for downstream applications?
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Upon creation of a cDNA library, quantitative qPCR or microarray analysis may be performed
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Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used to amplify archival fixed tissue or degraded samples?
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Yes, the WTA kit effectively amplifies degraded RNA, including formalin-fixed and paraffin-embedded samples.
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Is the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, also applicable for ribosomal RNA and/or small RNA amplification?
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Using the kit as is, Ribosomal RNA does appear to amplify. However, we have not quantitated how well it is amplified.
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What is the difference between WTA and WGA? I use the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1.
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The starting material for WTA is RNA, whereas for WGA it is genomic DNA. After the RNA is reverse-transcribed into the DNA complement by WTA, the OmniPlex library is formed. This library is in turn amplified by PCR.
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What is the concentration of the dNTPs supplied with the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1?
1 answer-
The dNTP Mix (D6193) is supplied as 10 mM each.
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