hSUMO1-AMC is prepared via the conjugation of 7-amino-4-methylcoumarin (AMC) to the C-terminal Gly of hSUMO1. This conjugation quenches the intrinsic fluorescence of AMC. Upon incubation with a protease recognizing hSUMOs, such as Senp1 or Senp2, AMC is released and the increase in fluorescence at 460 nm (Exc. 380 nm) can be measured. The enhanced purity of hSUMO1-AMC compared to that of other commercial vendors leads to a greater signal to noise ratio. This protein contains no extraneous tags.
Physical form
Lyophilized powder
Preparation Note
Centrifuge the vial prior to opening.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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