SRP5009
CDK1/CyclinB1, active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
CCNB, CCNB1, CDC2
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
Assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
12-16 nmol/min·mg
mol wt
~58 kDa (CDK1)
~75 kDa (CyclinB1)
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CCNB1(891) , CDK1(983)
General description
The cyclin B1 (CCNB1) gene is mapped to human chromosome 5q13.2. The gene codes for a 62kDa protein containing a classical cyclin box domain. CCNB1 shuttles between the nucleus and cytoplasm until prophase begins. It is a member of the cyclin family and is expressed at low levels in normal cells. CDK1 (cyclin-dependent kinase 1) belongs to the Ser/Thr protein kinase family. The gene encoding it is localized on human chromosome 10q21.
Biochem/physiol Actions
The cyclin B1 (CCNB1) and cyclin-dependent kinase 1 (CDK1) complex plays a crucial role in the regulation of mitosis. Elevated expression of CCNB1 has been observed in various types of human cancers including breast, lung, colon, prostate and head and neck cancers. Thus, this protein can be considered as a potent target for cancer vaccines.In addition, CCNB1 also serves as a prognostic biomarker for estrogen-receptor-positive (ER+) breast cancer. CDK1 (cyclin-dependent kinase 1) controls various mitotic processes like reorganization of the cytoskeleton and segregation of chromosomes. It is essential for the multiplication of mammalian cells.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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CCNB1 is a prognostic biomarker for ER+ breast cancer
Kun Ding
Medical Hypotheses, 83(3) (2014)
Expression analysis of mitotic spindle checkpoint genes in breast carcinoma: role of NDC80/HEC1 in early breast tumorigenicity, and a two-gene signature for aneuploidy
Ivan B
Molecular Cancer (2011)
Recurrent CDK1 overexpression in laryngeal squamous cell carcinoma
K. Bednarek
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 37(8) (2016)
Importance of tuberin in carcinogenesis (Review)
Mingwei Jin
Oncology Letters (2017)
Soraya Játiva et al.
Cellular and molecular life sciences : CMLS, 76(18), 3601-3620 (2019-03-31)
Exit from mitosis and completion of cytokinesis require the inactivation of mitotic cyclin-dependent kinase (Cdk) activity. In budding yeast, Cdc14 phosphatase is a key mitotic regulator that is activated in anaphase to counteract Cdk activity. In metaphase, Cdc14 is kept
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