SRP2034

Topo I (N1-197) (NTD) human

recombinant, expressed in insect cells, ≥85% (SDS-PAGE)

• Synonym(s): TOPI
• UNSPSC Code: 12352202
• NACRES: NA.26

Properties

• biological source: human
• recombinant: expressed in insect cells
• Assay: ≥85% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~25.3 kDa
• packaging: pkg of 5 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 1000 μg/mL
• color: clear colorless
• NCBI accession no.: NM_003286
• UniProt accession no.: P11387
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... TOP1(7150)

Description

Biochem/physiol Actions

Human DNA topoisomerase I is the best studied of the DNA topoisomerase family. It catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. The N-terminal 214 amino acids of topoisomerase I comprise a highly charged N-terminal domain (NTD) involved in protein-protein interactions with a number of cellular proteins, including SV40 T antigen, nucleolin, SR proteins, p53 and other transcription factors.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable