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recombinant
expressed in baculovirus infected Sf9 cells
Assay
70-95% (SDS-PAGE)
form
frozen liquid
mol wt
~100 kDa
concentration
0.1 μg/μL
accession no.
NM_024776
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PEAK1(79834)
Application
Useful for kinase assay and Western blot.
Biochem/physiol Actions
Recombinant human PEAK1 (1127-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PEAK1 or Pseudopodium-enriched atypical kinase 1 is a protein tyrosine kinase which belongs to NKF3 kinase family and regulates the cytoskeleton and has been shown to be involved in cancer progression (1). PEAK1 promotes anchorage-independent growth in the MDA-MB-435 human cancer cell line and promotes tumor progression of XPA-1 human pancreatic cancer cells when transplanted into the pancreas of nude mice. PEAK1 is highly expressed in spleen, followed by heart, ovary, brain, lung, kidney, and fetal brain (2).
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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