R2404

Anti-Raf-1/c-Raf antibody, Mouse monoclonal

clone RNP1, purified from hybridoma cell culture

• Synonym(s): Anti-CMD1NN, Anti-CRAF, Anti-NS5, Anti-Raf-1, Anti-c-Raf
• MDL number: MFCD01092478
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: RNP1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~75 kDa
• species reactivity: rat, mouse, human
• concentration: ~2 mg/mL
• technique(s): capture ELISA: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 2.0-4.0 μg/mL using extracts of A431 cell line (human epidermoid carcinoma)
• isotype: IgG1
• UniProt accession no.: P04049
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RAF1(5894) ; mouse ... Raf1(110157) ; rat ... Raf1(24703)

Description

General description

Monoclonal Anti-Raf-1/c-Raf (mouse IgG1 isotype) is derived from the RNP1 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids 334-345 of human Raf-1.

Raf-1 is a serine/threonine kinase that is activated by tyrosine kinases and functions upstream of the MAPK-signaling pathway. Raf-1 inhibits Ras signaling and subsequently modulates cell proliferation and development. c-Raf expression has been linked to the pathogenesis of lung carcinoma.

Specificity

Monoclonal anti-Raf-1/c-Raf antibody recognizes human, rat and mouse Raf-1 (~75 kDa). The product may also detect Raf-1 degradation products as additional weaker bands at approx. 50 kDa.

Immunogen

Synthetic peptide corresponding to amino acids of human Raf-1.

Application

Monoclonal Anti-Raf-1/c-Raf antibody has been used in:

• capture enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry
• double immunofluorescence analysis

Biochem/physiol Actions

Raf-1 (rapidly accelerated fibrosarcoma) controls cell growth and differentiation. Its oncogenic form can initiate malignant transformation of cells. Raf-1 has a critical role in the Ras/MAP kinase signalling pathway, integrating mitogenic signals in mammalian cells from many growth factors, cytokines and oncogenes, e.g., epidermal growth factor (EGF), platelet derived growth factor (PDGF), insulin, interleukin-2 (IL-2) and IL-3, granulocyte macrophage colony-stimulating factor (GM-CSF) and CSF-1. The regulation of Raf-1 activity is a highly complex and involves multistep process. Inactive Raf-1 is found in the cytosol, and is constitutively associated with the chaperones heat shock protein 90 (Hsp90), p50, and the 14-3-3 protein. Raf-1 activity is regulated by autophosphorylation at threonine residues and by phosphorylation at multiple serine and tyrosine residues by a number of protein kinases, including protein kinase C (PKC), non-receptor tyrosine kinase (Src) and Janus kinase 2 (JAK2).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves