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MAK310

Sigma-Aldrich

Ammonia Assay Kit

sufficient for 200 fluorometric tests

Synonym(s):

Ammonia Quantitative Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

fluorometric

relevant disease(s)

genitourinary disorders; gastrointestinal diseases

storage temp.

−20°C

General description

Ammonia (NH3) or the ammonium ion (NH4 +) is found in the atmosphere, rainwater, soil, seawater, and volcanic areas. It is widely used as fertilizer, being an important source of nitrogen for living systems. It also plays a role in animal physiology. Ammonia is produced by amino acid metabolism and is converted to urea in the human liver through the urea cycle. Ammonia acts as a precursor for various other nitrogen compounds. It is a colorless gas with pungent odor and can be easily liquefied. It is also used as a feed supplement in livestock industry.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the quantitative determination of ammonia/ammonium concentration in biological samples, such as urine, and environmental samples

Principle

The Ammonia Assay Kit provides a simple and high throughput adaptable assay for quantitative determination of ammonia/ammonium ion concentration in biological samples, such as urine, and environmental samples. This assay is based on the o-phthalaldehyde method in which the reagent reacts with ammonia/ammonium ion producing a fluorometric result ( ex = 360/ em = 450 nm), proportional to the ammonia concentration in the sample. The kit has a linear detection range of 0.012–1 mM ammonia in a 96 well format.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Daphné Coache et al.
PloS one, 17(6), e0270206-e0270206 (2022-06-25)
This study assessed the stability of six extemporaneously compounded hydroxyurea oral liquids stored at room temperature. Hydroxyurea oral liquids (100 mg/mL) were prepared using three different mixing methods (mortar, mixer or QuartetRx) from either bulk powder, capsule content, or whole
Umarah Mubeen et al.
The Plant cell, 30(10), 2240-2254 (2018-09-20)
The Target of Rapamycin (TOR) kinase is a central regulator of growth and metabolism in all eukaryotic organisms, including animals, fungi, and plants. Even though the inputs and outputs of TOR signaling are well characterized for animals and fungi, our
Beatriz Vallejo et al.
Microbial cell factories, 19(1), 124-124 (2020-06-09)
Saccharomyces cerevisiae wine strains can develop stuck or sluggish fermentations when nutrients are scarce or suboptimal. Nutrient sensing and signaling pathways, such as PKA, TORC1 and Snf1, work coordinately to adapt growth and metabolism to the amount and balance of
Hazardous materials chemistry for emergency responders, 553-553 (2013)
Adam Armada-Moreira et al.
Advanced biosystems, 4(10), e2000139-e2000139 (2020-09-02)
Excitotoxicity is a cellular phenomenon that comprises the consequences of toxic actions of excitatory neurotransmitters, such as glutamate. This process is usually related to overproduction of reactive oxygen species (ROS) and ammonia (NH4+ ) toxicity. Platinum nanoparticle (Pt-NP)-based microreactors able

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