The calibrator is incubated under the same conditions as the sample, and the activity is calculated relative to the absorbance of the calibrator, so it doesn't matter what temperature is used for incubation.
MAK123
α-Glucosidase Activity Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
α-Glucosidase Test Kit
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1 KIT
€541.00
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1 KIT
€541.00
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; neurological disorders
storage temp.
−20°C
Gene Information
human ... GAA(2548)
mouse ... GAA(14387)
rat ... GAA(367562)
General description
Application
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the detection of α-glucosidase activity in biological samples.
Principle
In this assay,α-glucosidase activity is determined by a reaction in which α-glucosidase hydrolyzes p-nitrophenyl-α-D-glucopyranoside resulting in the formation of a colorimetric (405 nm) product, proportional to the α-glucosidase activity present
Storage Class Code
10 - Combustible liquids
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about cat # MAK123, in the the protocol, the samples are incubated either at room temperature or 37°C and then absorbance is measured after 20 min. Please indicate me the incubation time at RT and incubation time at 37C because kinetic is different at T
1 answer-
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Hi, I have a question about cat # MAK123, a-glucosidase activity assay kit, which is sufficient for 100 assays. Once the reagents are opened, can they be used at a later date? eg - am I able to open the kit and run samples over several days?
1 answer-
The component included in this kit may be refrozen for later use until the expiration date. Do not store refrigerated.
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