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L8262

Sigma-Aldrich

Lectin from Ulex europaeus (gorse, furze)

biotin conjugate, lyophilized powder

Synonym(s):

Ulex europaeus agglutinin, UEA-I

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

conjugate

biotin conjugate

Quality Level

200

form

lyophilized powder

potency

<50 μg/mL agglutination activity

composition

Protein, ~85% E1%/280

extent of labeling

~3 mol biotin per mol protein

storage temp.

2-8°C

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Application

Lectin from Ulex europaeus (gorse, furze) has been used:
  • as a Paneth cells marker in immunohistochemistry at 1:1,000 dilution
  • in double staining for endothelial cells for quantitation of blood vessels
  • in histochemical staining of bronchial epithelial cells

Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Packaging

Package size based on protein content.

Linkage

Conjugates are prepared from affinity purified lectin.

Physical form

Contains sodium citrate

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Tango et al.
Archives of histology and cytology, 63(1), 81-89 (2000-04-19)
The aggregation of lymphoid cells in the bronchial mucosa has been named the bronchus-associated lymphoid tissue (BALT) and investigated in comparison with the gut-associated lymphoid tissue (GALT). To elucidate precisely the structure and function of the BALT, the present study
Maria Hernandez et al.
Biomedicines, 9(11) (2021-11-28)
The purpose of this study was to assess vascular and histological alterations in two COVID-19 and three control post-mortem retinas. The macular areas of flat-mounted samples were processed for immunofluorescence. Lectin and collagen IV positive vessels were captured under confocal
Daisuke Kondoh et al.
Cells, tissues, organs, 203(1), 20-28 (2016-07-18)
Glycoconjugates in the olfactory system play critical roles in neuronal formation, and α1-2 fucose (α1-2Fuc) glycan mediates neurite outgrowth and synaptic plasticity. Histochemical findings of α1-2Fuc glycan in the mouse olfactory system detected using Ulex europaeus agglutinin-I (UEA-I) vary. This
Sarah Waters et al.
Acta neuropathologica communications, 9(1), 144-144 (2021-08-28)
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease involving progressive degeneration of upper and lower motor neurons. The pattern of lower motor neuron loss along the spinal cord follows the pattern of deposition of phosphorylated TDP-43 aggregates. The blood-spinal
Heather Ireland et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 233(4), 1332-1336 (2005-06-07)
Here, we exploit an absolute differential between stem and progeny cells in their ability to express Cre from a somatically inducible transgene to determine the longevity of intestinal Paneth cells. In the Ahcre transgenic line induction of Cre recombinase allows
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