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L4268

Sigma-Aldrich

Lipopolysaccharides from Klebsiella pneumoniae

Lipopolysaccharides from Klebsiella pneumoniae

purified by phenol extraction

Synonym(s):

LPS

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10 MG
€131.00
100 MG
€777.00

€131.00

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10 MG
€131.00
100 MG
€777.00

About This Item

MDL number:
MFCD00131520
UNSPSC Code:
12352201
NACRES:
NA.25

€131.00

Please contact Customer Service for Availability
Request a Bulk Order

biological source

bacterial (Klebsiella pneumoniae)

Quality Level

200

form

lyophilized powder

purified by

phenol extraction

impurities

<3% Protein (Lowry)

color

white to faint yellow

solubility

water: 4.90-5.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

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General description

This product is phenol extracted from Klebsiella pneumoniae. The source strain is ATCC 15380.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000410664
0000410664
0000362509
0000312728
0000312727

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C Arnold Spek et al.
Cells, 10(9) (2021-09-29)
CCAAT/enhancer-binding protein delta (C/EBPδ) is a member of the C/EBP family of transcription factors. According to the current paradigm, C/EBPδ potentiates cytokine production and modulates macrophage function thereby enhancing the inflammatory response. Remarkably, however, C/EBPδ deficiency does not consistently lead
Max C Jacobs et al.
PloS one, 15(11), e0241748-e0241748 (2020-11-05)
An increasing body of evidence is indicating that the gut microbiota modulates pulmonary inflammatory responses. This so-called gut-lung axis might be of importance in a whole spectrum of inflammatory pulmonary diseases such as acute respiratory distress syndrome, chronic obstructive pulmonary
Sofia Monteiro et al.
Gut, 70(2), 379-387 (2020-04-04)
Systemic inflammation predisposes acutely decompensated (AD) cirrhosis to the development of acute-on-chronic liver failure (ACLF). Supportive treatment can improve AD patients, becoming recompensated. Little is known about the outcome of patients recompensated after AD. We hypothesise that different inflammasome activation
Nora S Wolff et al.
Intensive care medicine experimental, 8(1), 47-47 (2020-08-26)
The microbiome has emerged as an important player in the pathophysiology of a whole spectrum of diseases that affect the critically ill. We hypothesized that differences in microbiota composition across vendors can influence murine models of pulmonary lipopolysaccharide (LPS) inflammation
Teresa García-Lezana et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 37(3), 927-937 (2016-05-08)
Hepatic encephalopathy has traditionally been considered a reversible disorder. However, recent studies suggested that repeated episodes of hepatic encephalopathy cause persistent impairment leading to neuronal loss. The aims of our study were the development of a new animal model that
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Lipopolysaccharides for Research – Structure, Function & Applications

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Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

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Questions

  1. Is the product LPS from K. pneumoniae Cat N L4268 Ultrapure? As prevention to minimisation effect of the mixture in cell culture

    1 answer

    1. This product is purified by phenol extraction and is not considered ‘Ultrapure’. Currently, the term ‘Ultrapure’ is not assigned to any of the LPS products. However, the method of purification is highly indicative of the type and amount of contaminants that may be present. Of the various methods of preparation and purification available, the most pure products are those purified by ion exchange chromatography followed by those purified by gel filtration. Unfortunately, a chromatography purified version of LPS from Klebsiella is not currently available.
      Please see the link below for more information on the results of this type of purification.
      https://www.sigmaaldrich.com/technical-documents/technical-article/research-and-disease-areas/cell-signaling/lipopolysaccharides

      Please see the link below to review LPS products sorted by the purification method:
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/research-and-disease-areas/immunology-research/lipopolysaccharides-for-research

      Please see the following link to review the general handling and usage information available for LSP products:
      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

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