GUSS
β-Glucuronidase Reporter Gene Staining Kit
Synonym(s):
β-Glucuronidase Staining Kit, Reporter Gene Kit, Staining Kit for Glucuronidase
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.32
Recommended Products
usage
kit sufficient for 100 histochemical assays
Quality Level
technique(s)
microbe id | staining: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.
Application
The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.
Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.
Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.
Features and Benefits
- Histochemical staining of plant tissues expressing the E. coli GUS enzyme
- Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
- GUS does not interfere with plant cell function or viability
Other Notes
The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.
Kit Components Only
Product No.
Description
- 2× Fixation Buffer 25 mL
- 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial
- Reagent A for gus staining kit 50 mL
- Reagent B for gus staining kit 200 μL
- Reagent C for gus staining kit 200 μL
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
11 - Combustible Solids
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The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics.
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We have used the Escherichia coli beta-glucuronidase gene (GUS) as a gene fusion marker for analysis of gene expression in transformed plants. Higher plants tested lack intrinsic beta-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. We
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