GE17-0599-01

Sephacryl^® S-300 HR

Cytiva 17-0599-01, pack of 750 mL

• Synonym(s): Sephacryl S-300 Column
• UNSPSC Code: 41115709
• NACRES: NA.56

Properties

• packaging: pack of 750 mL
• manufacturer/tradename: Cytiva 17-0599-01
• matrix: allyl dextran and N, N’-methylenebisacrylamide
• particle size: 25-75 μm
• avg. part. size: 50 μm
• cleaning in place: 2-13
• working range: 3-11
• suitability: suitable for bioprocess medium

Description

General description

Sephacryl^® High Resolution gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.

Sephacryl^® High Resolution chromatography media is a highly versatile gel filtration media that offers a wide range of fractionation capabilities. The matrix of Sephacryl^® High Resolution media is a cross-linked copolymer of allyl dextran and N,N′-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The narrow particle size distribution, together with steep selectivity curves, results in good preparative characteristics with maintained high resolution. The hydrophilic nature of the media minimizes nonspecific adsorption and maximizes recovery. The excellent resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl^® HR the medium of choice for routine purification.

Sephacryl^® High Resolution is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As a BioProcess medium, Sephacryl^® High Resolution, meets industrial demands with security of supply and comprehensive technical and regulatory support.

Five different Sephacryl^® High Resolution chromatography media, with different fractionation ranges, are available:
Sephacryl^® S-100 High Resolution is designed for separating peptides and small proteins (fractionation range globular proteins 1 × 103–1 × 105).
Sephacryl^® S-200 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 103–2.5 × 105).
Sephacryl^® S-300 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 1 × 104–1.5 × 106).
Sephacryl^® S-400 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids. (fractionation range globular proteins 2 × 104–8 × 106).
Sephacryl^® S-500 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids (fractionation range Dextrans 4 × 104–2 × 107)

Features and Benefits

• Cost-efficient gel filtration media providing high resolution with fast and easy operation.
• Five different fractionation ranges available in Sephacryl^® HR family allows for reproducible separations over a wide molecular weight range.
• Sephacryl^® S-300 High Resolution medium is designed for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 1 × 104–1.5 × 106)
• BioProcess medium supported for industrial applications and well-established in approved processes.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sephacryl is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids