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G4410

Sigma-Aldrich

Griess reagent (modified)

Synonym(s):

Greiss, nitrite assay

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About This Item

EC Number:
UNSPSC Code:
12352200
NACRES:
NA.32

form

powder

usage

10 g

General description

Griess reagent is based on Griess reaction. The components include naphthylethylenediamine dihydrochloride suspended in water and sulphanilamide in phosphoric acid. This reagent reacts with nitrite in samples to form a purple azo product, absorbance of which is measured at 546 nm.

Application

Griess reagent (modified) has been used:
  • in the nitrite assay of primary spinal cord glial cells stimulated with supernatant of damaged sensory neuron (SDSN)
  • for measuring nitrite/nitrate oxidized from nitric oxide in stimulated chondrocytes
  • for measuring nitric oxide (NO) degradation in RAW264.7 macrophage cells
  • to measure the concentration of nitric oxide(NO) in the conditioned media from cultured macrophages (MΦs)
  • to determine the relative levels of NO released by BEAS-2B bronchial cells in the culture supernatants

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 2


Certificates of Analysis (COA)

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Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids
Green LC, et al.
Analytical biochemistry, 126(1), 131-138 (1982)
The C-terminal sequence of several human serine proteases encodes host defense functions
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Journal of Innate Immunity, 3(5), 471-471 (2011)
A critical role of toll-like receptor 2 in nerve injury-induced spinal cord glial cell activation and pain hypersensitivity
Kim D, et al.
The Journal of Biological Chemistry, 282(20), 14975-14983 (2007)

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