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293-PERV-PK-CIRCE

97051411, human kidney, Not specified

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About This Item

UNSPSC Code:
41106514

product name

293-PERV-PK-CIRCE, 97051411

biological source

human kidney

growth mode

Adherent aggregates

karyotype

Not specified

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human embryo kidney, infected with endogenous retrovirus of pigs

Cell Line Description

293-PERV-PK-CIRCE has been established by infecting the human kidney 293 cell line (Sigma Catalogue number. 85120602) with retrovirus produced by the pig kidney cell line PK-15. The endogenous, C-type retrovirus particles produced by PK-15 have been shown to be capable of infection, replication and serial transfer. 293-PERV-PK-CIRCE is useful to examine the host range of the pig retrovirus to assess the risks associated with porcine xenotransplantation. The cell line has tested negative for Hog Cholera virus. Handle under ACDP Category 2 conditions.

Application

Pig retrovirus studies, infectivity assays

Culture Medium

DMEM + 2mM Glutamine + 10% HI-FBS

Subculture Routine

Split sub-confluent cultures (70-80%) 1:6 cells can be dislodged by mechanical agitation 5% CO2 3

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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