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KNRK

87061001, rat kidney, Epithelial-like

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About This Item

UNSPSC Code:
41106514

product name

KNRK, 87061001

biological source

rat kidney

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial-like

products

Growth factors

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Normal rat kidney, Kirsten MSV transformed

Cell Line Description

Derived from normal rat kidney transformed by Kirsten sarcoma virus. Cells do not produce virus but carry the virus genome. In serum free medium the cell line produces growth factors capable of stimulating quiescent cells to grow in monolayers or in soft agar.

Application

Transformation, growth factors and virus studies: Kirsten MSV

Culture Medium

DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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