W6/1

13082201, from Mouse x Mouse Hybridoma

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myeloma: P3-NS1/1-Ag4.1
• Reactivity: Human ′A′ erythrocytes
• technique(s): cell culture | mammalian: suitable
• isotype: IgM
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Description

The W6/1 hybridoma cell line secretes monoclonal IgM antibodies reactive with a polymorphic determinant of human erythrocytes which correlated with blood group A.

Immunogen

Human tonsil membrane

Application

Human erythrocyte marker.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subculture Routine

Seed at 5 x 10⁴ cells/ml. Subculture when cells reach 5 x 10⁵ -1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. Start in 20% FBS when recovering from frozen.

Other Notes

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.