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A3653

Sigma-Aldrich

Actin from bovine muscle

lyophilized powder

Synonym(s):

G-Actin

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About This Item

CAS Number:
51005-14-2
MDL number:
MFCD00130338
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

bovine muscle

Quality Level

200

Assay

≥90% (SDS-GE)

form

lyophilized powder

mol wt

43 kDa

composition

Protein, ≥80% biuret

technique(s)

western blot: suitable

UniProt accession no.

P62739

storage temp.

2-8°C

Gene Information

bovine ... LOC(528168)

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General description

Actin is a highly abundant and conserved protein. It can transition between the monomeric (G-actin) and filamentous (F-actin) forms, which is influenced by external factors. Actin is expressed in smooth, skeletal and cardiac muscles.

Application

Actin from bovine muscle has been used as an internal standard and positive control during western blotting.

Biochem/physiol Actions

Actin is involved in a wide variety of cellular processes like cell migration, phagocytosis, cell polarity and shape. It associates with myosin and aids in muscle contraction. The protein acts as a building block of microfilaments.

Physical form

Lyophilized powder containing Tris, ATP and CaCl2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Laure Diancourt et al.
Toxins, 11(3) (2019-03-03)
Clostridiumperfringens type E is a less frequently isolated C.perfringens type and has not previously been reported in France. We have characterized two recent type E isolates, C.perfringens 508.17 from the intestinal content of a calf that died of enterotoxemia, and
T V L Berghof et al.
Poultry science, 89(2), 227-235 (2010-01-16)
Natural antibodies (NAb) have been divided in 2 classes: overt and cryptic. Overt NAb can be detected in unfractionated normal sera of nonimmunized mammals and chickens. Cryptic NAb as described in mammals need an in vitro physical or biochemical treatment
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At high concentrations, selenium (Se) exerts phytotoxic effects in non-tolerant plant species partly due to the induction of nitro-oxidative stress; however, these processes are not fully understood. In order to obtain a more accurate view of the involvement of nitro-oxidative
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Articles

Actin Protein

Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.

Actin Protein

Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.

Actin Protein

Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.

Actin Protein

Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.

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