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60035

Sigma-Aldrich

Potassium acetate

BioUltra, for molecular biology, ≥99.0% (NT)

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About This Item

Linear Formula:
CH3COOK
CAS Number:
Molecular Weight:
98.14
Beilstein:
3595449
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

product line

BioUltra

Assay

≥99.0% (NT)

form

powder or crystals

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

7.0-9.0 (25 °C, 1 M in H2O)

solubility

H2O: 1 M at 20 °C, clear, colorless

density

1.57 g/cm3 at 25 °C (lit.)

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
Li: ≤5 mg/kg
Mg: ≤10 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤5000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.040
λ: 280 nm Amax: ≤0.020

SMILES string

[K+].CC([O-])=O

InChI

1S/C2H4O2.K/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1

InChI key

SCVFZCLFOSHCOH-UHFFFAOYSA-M

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Application

Potassium acetate is used in molecular biology to precipitate sodium dodecyl sulfate and SDS-bound proteins that result from SDS extraction protocols. Potassium acetate facilitates the purification of DNA when using SDS protocols and ethanol DNA precipitation protocols.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Steady-state and time-resolved emission techniques were employed to study the effect of acetate, a mild base, on the luminescence of curcumin in methanol and ethanol. We found that the steady-state emission intensity as well as the average fluorescence decay time
Fátima Gebauer et al.
Methods in enzymology, 429, 23-33 (2007-10-05)
Classically, Drosophila cell-free translation systems have been used to study the response of the translational machinery to heat shock treatment. We and others have developed optimized Drosophila embryo and ovary extracts, and their use has expanded to the study of
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The stable isotopes of organic matter can provide valuable information on carbon cycling dynamics, microbial metabolisms, and past climates. Since bulk measurements may mask dynamic changes to critical portions of the organic pool, researchers are increasingly isolating individual compounds for
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The Journal of biological chemistry, 279(29), 30307-30315 (2004-05-13)
The yeast open reading frames YOL027 and YPR125 and their orthologs in various eukaryotes encode proteins with a single predicted trans-membrane domain ranging in molecular mass from 45 to 85 kDa. Hemizygous deletion of their human homolog LETM1 is likely

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