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40809

Sigma-Aldrich

Purine nucleoside phosphorylase

recombinant, expressed in E. coli, ≥120 U/mL

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About This Item

CAS Number:
9030-21-1
Enzyme Commission number:
2.4.2.1 (BRENDA, IUBMB)
MDL number:
MFCD00131739
UNSPSC Code:
12352204

recombinant

expressed in E. coli

form

buffered aqueous solution (100 mM phosphate buffer with 1 mM MgCl2)

specific activity

≥120 U/mL

storage temp.

−20°C

InChI

1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1

InChI key

MRWXACSTFXYYMV-FDDDBJFASA-N

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Application

Purine nucleoside phosphorylase is used to study nucleotide salvage pathways and purine metabolism[1]. It is used to study purine nucleoside phosphorylase deficiency and responsible PNP genetic mutations[2].

Biochem/physiol Actions

Purine nucleoside phosphorylase is an enzyme involved in purine metabolism. PNP metabolizes adenosine into adenine, inosine into hypoxanthine, and guanosine into guanine[1]. Mutations in the PNP gene are responsible for purine nucleoside phosphorylase deficiency[2].

Unit Definition

1 U will cause the phosphorolysis 1 μmol of inosine to hypoxanthine and ribose 1-phosphate per minute at pH 7.4 at 25°C

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCBQ2936V
BCBN7658V
BCBL2387V
BCBJ7734V
BCBD8998

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Purine nucleoside phosphorylase deficiency with a novel PNP gene mutation: a first case report from India
Manisha Rajan Madkaikar, et al.
BMJ Case Reports, DOI:10-DOI:10 (2011)
Fernanda Canduri et al.
Biochemical and biophysical research communications, 313(4), 907-914 (2004-01-07)
Human purine nucleoside phosphorylase (PNP) is a ubiquitous enzyme which plays a key role in the purine salvage pathway, and PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effect on B-cell function. PNP
M Palmer et al.
Alimentary pharmacology & therapeutics, 36(4), 370-378 (2012-06-20)
Combination therapy with the ribavirin (RBV) prodrug taribavirin (TBV) and pegylated interferon (PIFN) has produced lower rates of anaemia than with RBV and PIFN. Studies have demonstrated that the sharpest decline in viral load during TBV therapy occurs at Weeks
Candida Deves et al.
Bone, 52(1), 167-175 (2012-10-03)
Purine nucleoside phosphorylase (PNP) is a purine-metabolizing enzyme that catalyzes the reversible phosphorolysis of 6-oxypurine (deoxy)nucleosides to their respective bases and (deoxy)ribose-1-phosphate. It is a key enzyme in the purine salvage pathway of mammalian cells. The present investigation sought to
K K Jason Chan et al.
Methods in enzymology, 516, 219-235 (2012-10-05)
Nature has hardly evolved a biochemistry of fluorine although there is a low-level occurrence of fluoroacetate found in selected tropical and subtropical plants. This compound, which is generally produced in low concentrations, has been identified in the plants due to
View All Related Papers

Questions

1–2 of 2 Questions  

  1. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. What is the source gene of the purine nucleoside phosphorylase, Product 40809?

    1 answer

    1. Per our supplier, the purine nucleoside phosphorylase,product 40809, is of bacterial origin, specifically from Enterobacter.

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