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09341

Sigma-Aldrich

8-Aminopyrene-1,3,6-trisulfonic acid trisodium salt

suitable for fluorescence, BioReagent, ≥96.0% (HPCE)

Synonym(s):

1,3,6-Pyrenetrisulfonic acid, 8-amino trisodium salt, APTS, Trisodium 8-aminopyrene-1,3,6-trisulfonate

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About This Item

Empirical Formula (Hill Notation):
C16H8NNa3O9S3
CAS Number:
196504-57-1
Molecular Weight:
523.40
MDL number:
MFCD00674494
UNSPSC Code:
12352106
PubChem Substance ID:
329748761
NACRES:
NA.32

product line

BioReagent

Assay

≥96.0% (HPCE)

mp

≥250 °C (lit.)

solubility

DMF: soluble
DMSO: soluble
H2O: soluble

fluorescence

λex 420 nm; λem 500 nm in 0.1 M Tris pH 7.4 (quenching with Vitamin B1)
λex 488 nm; λem 520 nm(lit.)

suitability

suitable for fluorescence

SMILES string

[Na+].[Na+].[Na+].Nc1cc(c2ccc3c(cc(c4ccc1c2c34)S([O-])(=O)=O)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H11NO9S3.3Na/c17-11-5-12(27(18,19)20)8-3-4-10-14(29(24,25)26)6-13(28(21,22)23)9-2-1-7(11)15(8)16(9)10;;;/h1-6H,17H2,(H,18,19,20)(H,21,22,23)(H,24,25,26);;;/q;3*+1/p-3

InChI key

XSTNYACEWLNWPY-UHFFFAOYSA-K

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Application

APTS is a fluorescence reagent that has been demonstrated to have a high level of detection sensitivity for sugars The LOD for APTS-derivatized sugars has been shown to be 0.4 nM when using a 488 nm argon-ion laser. The multi-anionic nature of APTS makes it ideal for the studies of carbohydrate molecules and small hormone molecules by high resolution capillary electrophoresis.
Fluorescent label for saccharides and glycoproteins.

Other Notes

Fluorescent probe for derivatising sugars

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Meininger et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1012-1013, 193-203 (2016-02-07)
Recombinant human erythropoietin (rhEPO) is an important CHO cell-derived glycoprotein and the degree of sialylation of this hormone is crucial for its in vivo bioactivity. In order to improve the purification process serotonin as a potential affinity ligand was tested
R.A. Evangelista et al.
Analytical Chemistry, 67, 2239-2239 (1995)
H Suzuki et al.
Analytical chemistry, 69(22), 4554-4559 (1998-01-07)
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), has been used for structure characterization of 1-aminopyrene-3,6,8-trisulfonate (APTS)-derivatized oligosaccharides previously separated by capillary electrophoresis (CE). The resolved components were first isolated by employing an automated high-resolution fraction collector. Using on-probe sample
Jennifer Dorcas Hwang et al.
Journal of laboratory automation, 21(1), 153-165 (2015-07-15)
Industrial enzymes are instrumental in many applications, including carbohydrate processing, fabric and household care, biofuels, food, and animal nutrition, among others. Enzymes have to be active and stable not only in harsh application conditions, but also during shipment and storage.
René Hennig et al.
Biochimica et biophysica acta, 1860(8), 1728-1738 (2016-04-04)
Facilitated by substantial advances in analytical methods, plasma N-glycans have emerged as potential candidates for biomarkers. In the recent years, several investigations could link aberrant plasma N-glycosylation to numerous diseases. However, due to often limited specificity and sensitivity, only a
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