Skip to Content
Merck
All Photos(1)

Documents

M0428

Millipore

Middlebrook 7H11 Agar Base

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Media for culturing Mycobacteria

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41171609
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

storage condition

protect from light at room temperature

technique(s)

microbe id | susceptibility testing: suitable
microbiological culture: suitable

final pH

6.6±0.2 (25 °C)

application(s)

clinical testing
food and beverages
life science and biopharma
veterinary
microbiology

suitability

mycobacteria

Related Categories

General description

Middlebrook 7H11 Agar Base media is used for the isolation, cultivation, and sensitivity testing of Mycobacterium species. This agar base contains many inorganic salts which help the growth of Mycobacteria. Citric acid formed from sodium citrate helps in retaining inorganic cations in solution. Glycerol supplies carbon and energy. The Middlebrook OADC Supplement (M0678) contains oleic acid, bovine albumin, sodium chloride, dextrose and catalase. Oleic acid and other long chain fatty acids are essential for the metabolism of Mycobacteria. Dextrose is an energy source. Catalase neutralizes toxic peroxides. Albumin protects tubercle bacilli from toxic agents. Malachite green partially inhibits other bacteria.

Application

There are two types of solid culture media for the primary isolation of Mycobacteria. Egg-based (Lowenstein’s formulation) and agar-based (Middlebrook formulation)
The latter has the following advantages over the egg-based media

The Middlebrook 7H11 agar base does not liquify easily in the presence of contaminating proteolytic organisms

There is less inactivation of added drugs when egg ingredients are absent

Components

Ingredients (g/L)
Casein enzymatic hydrolysate, 1.00
Ammonium sulfate, 0.50
Monopotassium phosphate, 1.50
Disodium phosphate, 1.50
Sodium citrate, 0.40
Magnesium sulfate, 0.05
L-Glutamic acid, 0.50
Ferric ammonium citrate, 0.04
Pyridoxine, 0.001
Biotin, 0.0005
Malachite green, 0.001
Agar, 15.00

Preparation Note

Suspend 10.25 g of Middlebrook 7H11 Agar Base in 450 ml of distilled water. Add 2.5 ml of glycerol (Cat. No. 49767). Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 1 vial Middlebrook OADC Growth Supplement (M0678). Mix thoroughly before dispensing.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCL3712
BCCM1237
BCCK1926
BCCH8643
BCCG8003

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Shuxin Liang et al.
Autophagy, 16(8), 1453-1467 (2019-11-13)
BAG2 (BCL2 associated athanogene 2) is associated with cell fate determination in response to various pathological conditions. However, the effects of BAG2 on M. tuberculosis-induced endoplasmic reticulum (ER) stress remain elusive. Herein, we report that M. tuberculosis infection of macrophages
Thomas R Lerner et al.
The Journal of clinical investigation, 126(3), 1093-1108 (2016-02-24)
In extrapulmonary tuberculosis, the most common site of infection is within the lymphatic system, and there is growing recognition that lymphatic endothelial cells (LECs) are involved in immune function. Here, we identified LECs, which line the lymphatic vessels, as a
Antony Fearns et al.
PLoS biology, 18(12), e3000879-e3000879 (2021-01-01)
Correlative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, we developed correlative light, electron
MacFaddin, J
Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, 1 (1985)

Articles

Detection & Molecular Identification of Mycobacteria

Identifying Mycobacterium species crucial due to varying clinical significance; some pathogenic, others not.

Detection & Molecular Identification of Mycobacteria

Identifying Mycobacterium species crucial due to varying clinical significance; some pathogenic, others not.

Detection & Molecular Identification of Mycobacteria

Identifying Mycobacterium species crucial due to varying clinical significance; some pathogenic, others not.

Detection & Molecular Identification of Mycobacteria

Identifying Mycobacterium species crucial due to varying clinical significance; some pathogenic, others not.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service