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Sigma-Aldrich

Neutral Red

for microscopy (Bact., Bot., Fl., Hist., Vit.), indicator (pH 6.8-8.0)

Synonym(s):

3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red

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About This Item

Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
553-24-2
Molecular Weight:
288.78
Colour Index Number:
50040
Beilstein:
3918740
EC Number:
209-035-8
MDL number:
MFCD00012651
UNSPSC Code:
12171500
PubChem Substance ID:
329763896
NACRES:
MA.02

grade

for microscopy (Bact., Bot., Fl., Hist., Vit.)
indicator (pH 6.8-8.0)

Quality Level

100

form

powder or crystals

technique(s)

titration: suitable

pH

6.8-8.0, red to yellow

mp

290 °C (dec.) (lit.)

solubility

water: 0.01 g/10 mL

εmax

≥800 at 535-545 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

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General description

Neutral red is a vital dye widely used as a cellular pH indicator and is selective for shifts in pH. It is highly lipophilic in nature. It favours membrane potential dependent fluorescence that attributes to changes in hydrophobicity.

Application

Neutral red has been used to stain mouse mammary glands.
Useful as an indicator for preparing neutral red paper, and as a biological stain.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK5416
BCCH5901
BCCF1209
BCCD3079
BCCB9483

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John D Landua et al.
Journal of mammary gland biology and neoplasia, 14(4), 411-415 (2009-11-26)
Whole mount preparations of mouse mammary glands are useful for evaluating overall changes in growth and morphology, and are essential for detecting and evaluating focal or regionally-localized phenotypes that would be difficult to detect or analyze using other techniques. We
Sylvie V M Tesson et al.
Frontiers in microbiology, 9, 2681-2681 (2018-11-30)
Microalgae are common members of the atmospheric microbial assemblages. Diverse airborne microorganisms are known to produce ice nucleation active (INA) compounds, which catalyze cloud and rain formation, and thus alter cloud properties and their own deposition patterns. While the role
Ayesha Ali et al.
Cell reports, 30(5), 1463-1477 (2020-02-06)
The intact vaginal epithelium is essential for women's reproductive health and provides protection against HIV and sexually transmitted infections. How this epithelium maintains itself remains poorly understood. Here, we used single-cell RNA sequencing (RNA-seq) to define the diverse cell populations
Gennady A Evtugyn et al.
Talanta, 102, 156-163 (2012-11-28)
New aptasensors based on DNA aptamer and polycarboxylated thiacalix[4]arenes in cone, 1,3-alternate and partial cone configurations bearing Neutral Red (NR) at substituents at the lower rim have been developed and applied for thrombin detection. The assembly of the biorecognition layer
Mara Ceridono et al.
Regulatory toxicology and pharmacology : RTP, 63(3), 480-488 (2012-06-13)
This is the report from the "ECVAM-EFPIA workshop on 3T3 NRU Phototoxicity Test: Practical Experience and Implications for Phototoxicity Testing", jointly organized by ECVAM and EFPIA and held on the 25-27 October 2010 in Somma Lombardo, Italy. The European Centre
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