49971

D-Lactic Dehydrogenase from Lactobacillus leichmannii

suspension, yellow, ~1000 U/mL

• Synonym(s): (R)-Lactate:NAD⁺ oxidoreductase, D-Lactate Dehydrogenase
• CAS Number: 9028-36-8
• Enzyme Commission number: 1.1.1.28 (BRENDA, IUBMB)
• MDL number: MFCD00130907
• UNSPSC Code: 12352204

Properties

• biological source: bacterial (Lactobacillus leichmannii)
• form: suspension
• specific activity: ≥30 U/mg protein
• concentration: ~1000 U/mL
• impurities: L-Lactat Dehydrogenase, none detected; 3.2 M Ammonium sulfate solution (pH ~6.2)
• color: yellow
• storage temp.: 2-8°C

Description

Application

In the food industry, the primary catalysis is coupled to conversion of NADH and H⁺ to NAD⁺ with diaphorase coupled with converting the non-fluorescent resazurin to the highly fluorescent substance resorufin to measure the content of D-lactate in food products.

Biochem/physiol Actions

D-lactic dehydrogenase catalyzes the conversion of pyruvate into D-lactate, with oxidation of NADH to NAD⁺. D-lactic dehydrogenase can also catalyze the reverse reaction, conversion of D-lactate into pyruvate with reduction of NAD⁺ to NADH.

D-lactic dehydrogenase catalyzes the conversion of pyruvate into D-lactate, with oxidation of NADH to NAD⁺. D-lactic dehydrogenase can also catalyze the reverse reaction, conversion of D-lactate into pyruvate with reduction of NAD⁺ to NADH.

Unit Definition

1 U corresponds to the amount of enzyme which will reduce 1 μmol of pyruvate to D-lactate per minute at pH 7.0 and 25 °C

Physical form

only partially soluble in water or buffer

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves