Different lysis methods may have specific pH requirements. The pH is a secondary factor in nucleic acid binding to the silica, with the salts in the buffer being the primary driver of the silica's negative charge. While optimizing the pH can enhance yields, the recommended protocols should be ideal.
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About This Item
UNSPSC Code:
12352401
NACRES:
NA.21
Recommended Products
Analysis Note
Appearance: black suspension
Magnetic Response: 1 - 20 s
Settling time: ≥ 5 min
RNases: not detectable
Fe (Iron): ≤ 5 ppm
Particlesurface (BET): 14 - 25 m²/g
For research use only
Magnetic Response: 1 - 20 s
Settling time: ≥ 5 min
RNases: not detectable
Fe (Iron): ≤ 5 ppm
Particlesurface (BET): 14 - 25 m²/g
For research use only
Legal Information
MAGPREP is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Discover how MagPrep® Silica particles meet essential needs for DNA and RNA nucleic acid purification protocols.
Discover how MagPrep® Silica particles meet essential needs for DNA and RNA nucleic acid purification protocols.
Discover how MagPrep® Silica particles meet essential needs for DNA and RNA nucleic acid purification protocols.
Discover how MagPrep® Silica particles meet essential needs for DNA and RNA nucleic acid purification protocols.
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Please provide clarification on the pH during the lysis and binding steps for the MagPrep products
1 answer-
Helpful?
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What are the dimensions of the particles in product 1.01193, including the size of the magnetic core and the thickness of the silica coating?
1 answer-
MagPrep Silica particles have a diameter of 50-150 nm and are suspended in 50mM MES buffer at pH 5.5.
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