A highly sensitive quantitative method was developed to detect protons released or taken up upon ligand binding. A small change in pH due to proton release or uptake was detected by measuring the difference in the absorbance of a pH
Biochimica et biophysica acta, 727(1), 22-30 (1983-01-05)
The kinetics of H+/OH- diffusion across dimyristoyl phosphatidic acid bilayer membranes was measured by following the absorbance of the pH-sensitive indicator Cresol red (o-cresolsulfonphthalein) entrapped in single lamellar vesicles after rapidly changing the external pH in a stopped-flow apparatus. The
If buffer/indicator systems are used as optical thermometers in multicuvettes, the temperature resolution is limited by errors of optical measurements produced mainly by variations of pathlength and blank transmittance of the wells. Theoretical and practical approaches show that, in multicuvettes
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 67(2), 412-419 (2006-09-09)
The effects of cationic cetyltrimethylammonium bromide (CTAB), hexadecylpyridinum chloride (HDPC) and anionic sodium dodecylsulphate (SDS) surfactants on dissociation constants and transition intervals of methyl red, methyl orange and cresol red were studied spectrophotometrically. The results show that all studied indicators
The pKa of 3',3",5',5"tetrabromo-m-cresolsulfonephtalein (Bromocresol Green) and o-cresolsulphonephtalein (Cresol Red) was spectrophotometrically measured in a water/AOT/isooctane microemulsion in the presence of a series of buffers carrying different charges at different water/surfactant ratios. Extended Principal Component Analysis was used for a
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.