The same Amicon filter unit can be used for repeated spins with the same sample. However, repeated spins may lead to increased clogging, resulting in slower flow rates and longer spin times.
UFC9030
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 30 kDa
Synonym(s):
15 mL sample volume, 30 kDa MWCO, Centrifugal concentrator
Sign Into View Organizational & Contract Pricing
All Photos(3)
Select a Size
Change View
8 PKG
€146.00
24 PKG
€385.00
96 PKG
€1,470.00
About This Item
UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
material:
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
pore size:
30 kDa MWCO
L:
12.1 cm (4.807 in.)
diam.:
2.97 cm
filtration area:
7.6 cm2
volume:
15 mL
sterility:
non-sterile
Recommended Products
material
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
manufacturer/tradename
Amicon® Ultra
parameter
15 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
12.1 cm (4.807 in.)
diam.
2.97 cm
filtration area
7.6 cm2
volume
15 mL
pore size
30 kDa MWCO
shipped in
ambient
General description
Amicon® Ultra 15 mL centrifugal devices with Ultracel® 30,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Ovalbumin (45 kDa) at 1 mg/ml initial concentration is retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
Application
- Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipettor sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Physical form
Color Code: Clear with pink cap
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Articles
Filtration methods and membrane filter selection in nanoparticle purification and production.
Filtration methods and membrane filter selection in nanoparticle purification and production.
Filtration methods and membrane filter selection in nanoparticle purification and production.
Filtration methods and membrane filter selection in nanoparticle purification and production.
Protocols
Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.
Related Content
Purification methods for downstream analysis reduce sample complexity, remove interferences, and concentrate analytes for more accurate results.
Purification methods for downstream analysis reduce sample complexity, remove interferences, and concentrate analytes for more accurate results.
Purification methods for downstream analysis reduce sample complexity, remove interferences, and concentrate analytes for more accurate results.
Purification methods for downstream analysis reduce sample complexity, remove interferences, and concentrate analytes for more accurate results.
-
How many times can sample be added to the same filter unit? For example: If I have 100 mL cell free supernatant and want to add 12 mL at a time, centrifuge and collect retentate - followed by discarding the flow through and pouring the next 12 mL etc.
1 answer-
Helpful?
-
-
I noticed no volume change after centrifuging 10mL cell media at 14,000xg for 20minutes. Could this be due to unsuccessful concentration?
1 answer-
The lack of volume change suggests that the sample may contain a high amount of particulate matter, leading to rapid clogging and preventing effective concentration.
Helpful?
-
-
Hello! Our lab had to move to a different area of our building and we found some probably quite old boxes of Amicon Ultra 15s (Cat UFC903096, lot R8CN67048). Does this product have an expiration date/shelf life? If so, has this lot surpassed it?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.
For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
