RADPKMAG-80K

MILLIPLEX^® Rat Adipokine Panel - Metabolism Assay

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• species reactivity: rat
• Quality Level: 200
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 94-119%; sensitivity: 0.3-17 pg/mL; (MinDC); standard curve range: 0.7-3,000 pg/mL; (IL-1β, TNFα); standard curve range: 12-50,000 pg/mL; (PAI-1); standard curve range: 15-60,000 pg/mL; (IL-6, Leptin); standard curve range: 24-100,000 pg/mL; (Insulin); standard curve range: 9.8-40,000 pg/mL; (MCP-1)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

In the past, the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism.

MILLIPLEX^® Rat Adipokine Magnetic Bead Panel is a seven-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum and plasma samples: IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

The MILLIPLEX^® MAP Rat Adipokine Magnetic Bead Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1 Total, and TNFα.

Specificity

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Application

• Analytes: IL-1β, IL-6, Insulin, Leptin, MCP-1 (CCL2), PAI-1 (Total), TNFα
• Recommended Sample Type: Rat serum or plasma
• Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma
• Assay Run Time: Overnight (16-18) hours at 2-8°C
• Research Category: Metabolism
• Research Subcategory: Endocrine; Obesity; Metabolic Disease

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Components

Rat Adipokine Standard, 1 vial, lyophilized

Rat Adipokine Quality Control 1, 1 vial, lyophilized

Rat Adipokine Quality Control 2, 1 vial, lyophilized

Assay Buffer, 1 bottle, 30 mL

Bead Diluent, 1 vial, 3.5 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Rat Adipokine Detection Antibodies, 1 bottle, 5.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.

Sensitivity: Please see kit protocol for individual assay sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H317,H319,H411
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects