MABT1533

Anti-Actin Antibody, clone 5-16 H1C8

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• antibody form: purified antibody
• clone: MAb GEa (5-16 H1C8); monoclonal
• purified by: (Ligatrap)
• species reactivity: mouse, human
• concentration: (Please refer to lot specific datasheet.)
• technique(s): ELISA: suitable; western blot: suitable
• isotype: IgM
• UniProt accession no.: P0CJ46

Description

General description

Purified actin (ACT1) from Arabidopsis thaliana plant.

Specificity

Clone 5-16 H1C8 is a mouse monoclonal antibody that detects Actin in a wide range of species.

Application

ELISA Analysis: A representative lot detected Actin in ELISA applications (Kandasamy, M.K., et. al. (1999). Plant J. 18(6):681-91).

Western Blotting Analysis: A representative lot detected Actin in Western Blotting applications (Kandasamy, M.K., et. al. (2012). Plant Cell. 24(5):2041-57; Kandasamy, M.K., et. al. (1999). Plant J. 18(6):681-91).

Quality

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Actin in human skeletal muscle tissue lysate.

Physical properties

~44 kDa observed; 41.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Purified mouse monoclonal antibody IgM in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Other Notes

Human. Predicted to react with all eukaryotes based on 100% sequence homology.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.