MABN1727
Anti-Beta-IV spectrin Antibody, clone N393/76
clone N393/76, from mouse
Synonym(s):
Spectrin beta chain, non-erythrocytic 4, Spectrin, non-erythroid beta chain 3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
N393/76, monoclonal
species reactivity
human, rat
species reactivity (predicted by homology)
mouse (based on 100% sequence homology)
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... SPTBN1(6711)
General description
Spectrin beta chain, non-erythrocytic 4 (UniProt: Q9H254; also known as Beta-IV spectrin, Spectrin, non-erythroid beta chain 3) is encoded by the SPTBN4 (also known as KIAA1642, SPTBN3) gene (Gene ID: 57731) in human. Beta-IV Spectrin belongs to the spectrin family of proteins and is abundantly expressed in brain and pancreatic islets. It belongs to the spectrin family of proteins and contains two CH (calponin-homology) domains, one PH domain, and 18 spectrin repeats. It has been isolated as an interactor of the receptor tyrosine phosphatase ICA512. Spectrin molecule is assembled as a tetramer of two alpha and two beta subunits. A tight association between the amino-terminal domain of an alpha subunit and the carboxyl-terminal domain of a beta subunit forms a heterodimer. In brain, beta-IV Spectrin co-localizes with ankyrin-G 480/270-kDa at initial segments and nodes of Ranvier of myelinated neurons. In brain its highest expression is observed in the cell bodies of large myelinated neurons. It is not detected in non-neuronal cells, including glial and endothelial cells.Beta-IV Spectrin along with ankyrin-G is required for the anchoring of voltage-gated Na+ channels and for cell adhesion molecule anchoring to the actin cytoskeleton. (Ref.: Berghs, S et al. (2000). J. Cell Biol. 151(5): 985-1002).
Specificity
Clone N393/76 specifically detects human and rat beta-IV Spectrin by immunohistochemistry. it targets a sequence within 192 amino acids in the C-terminal half of human beta-IV Spectrin.
Immunogen
A recombinant fusion fragment corresponding to 192 amino acids from the C-terminal half of human beta-IV spectrin.
Application
Anti-Beta-IV spectrin Antibody, clone N393/76, Cat. No. MABN1727, is a highly specific mouse monoclonal antibody that targets beta-IV spectrin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected beta-IV spectrin in human pancreas, human cervical cancer, and human uterus tissue.
Western Blotting Analysis: A representative lot detected beta-IV spectrin in COS cells transiently transfected with Myc-tagged beta 4-spectrin.
Immunohistochemistry Analysis: A representative lot detected beta-IV spectrin in rat cerebral cortex and hippocampus tissues.
Western Blotting Analysis: A representative lot detected beta-IV spectrin in COS cells transiently transfected with Myc-tagged beta 4-spectrin.
Immunohistochemistry Analysis: A representative lot detected beta-IV spectrin in rat cerebral cortex and hippocampus tissues.
Research Category
Neuroscience
Neuroscience
Quality
Evaluated by Immunohistochemistry in human cerebellum tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected beta-IV spectrin in human cerebellum tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected beta-IV spectrin in human cerebellum tissue.
Target description
288.98 kDa calculated.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Certificates of Analysis (COA)
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Drew M Nassal et al.
The Journal of biological chemistry, 297(1), 100893-100893 (2021-06-22)
Fibrosis is a pronounced feature of heart disease and the result of dysregulated activation of resident cardiac fibroblasts (CFs). Recent work identified stress-induced degradation of the cytoskeletal protein βIV-spectrin as an important step in CF activation and cardiac fibrosis. Furthermore
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