biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies, primary antibodies
clone
3G6.4.8, monoclonal
mol wt
calculated mol wt 60.18 kDa
observed mol wt ~37 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100 μg
technique(s)
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... CSF1(1435)
Related Categories
General description
Macrophage colony-stimulating factor 1 (UniProt: P09603; also known as CSF-1, M-CSF, MCSF, Lanimostim) is encoded by the CSF1 gene (Gene ID: 1435) in human. M-CSF is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-32), which is subsequently cleaved off to generate the mature form that contains a lumenal domain (aa 33-496), a transmembrane domain (aa 497-517), and a cytoplasmic domain (aa 518-554). It can be present as a disulfide-linked homodimer or heterodimer. Two distinct biologically active forms of M-CSF have been described: a membrane-bound and a secreted form that result from alternative splicing of the CSF1 gene. It plays an essential role in the regulation of survival, proliferation, and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. It promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. M-CSF is also involved in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and for normal bone development. Its biological effects are mediated via binding to CSF-1R/c-Fms receptor that is expressed by multipotent hematopoietic cells, mononuclear phagocyte progenitor cells, monocytes, tissue macrophages, and osteoclasts. Aberrant expression of M-CSF or CSF-1R is reported to promote cancer cell proliferation, invasion and formation of metastases. Overexpression of M-CSF or CSF-1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers.
Specificity
Clone 3G6.4.8 is a mouse monoclonal antibody that detects human macrophage colony-stimulating factor 1 (M-CSF). It targets an epitope within the N-terminal half.
Immunogen
A recombinant fragment corresponding to 149 amino acids from the N-terminal half of human Macrophage colony-stimulating factor 1 (M-CSF) with a GHERQSEGS sequence attached at its C-terminal end.
Application
Quality Control Testing
Evaluated by Western Blotting with recombinant Human M-CSF.
Western Blotting Analysis (WB): A 1:100,000 dilution of this antibody detected recombinant human M-CSF under non-denaturing conditions.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected M-CSF in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with recombinant Human M-CSF.
Western Blotting Analysis (WB): A 1:100,000 dilution of this antibody detected recombinant human M-CSF under non-denaturing conditions.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected M-CSF in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-M-CSF, clone 3G6.4.8, Cat. No. MABF3177, is a mouse monoclonal antibody that detects M-CSF and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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