MAB4418
Anti-Achaete Scute homolog 2 Antibody, clone 7E2
clone 7E2, from mouse
Synonym(s):
achaete-scute complex (Drosophila) homolog-like 2, achaete-scute complex homolog 2 (Drosophila), achaete-scute complex homolog-like 2, achaete-scute complex-like 2, achaete-scute complex-like 2 (Drosophila), mammalian achaete/scute homologue 2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
7E2, monoclonal
species reactivity
human
technique(s)
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ASCL2(430)
General description
Achaete Scute homolog 2 (also known as mammalian aschaete-scute homolog 2 or Mash2) is a nuclear protein expressed specifically in the extravillous trophoblasts of developing placenta. Placental development involves control by the basic helix-loop-helix transcription factor Achaete Scute homolog 2, which may regulate HIF (hypoxia) in the formation of spongiotrophoblasts. Targeted mutagenesis of Achaete Scute homolog 2 yielded loss of function results in embryonic lethality at midgestation due to placental failure associated with a lack of spongiotrophoblasts and reduced labyrinthine trophoblast layers. In addition to healthy placental development, Achaete Scute proteins may also be involved in the determination of neuronal precursors in the central and peripheral nervous systems. Achaete Scute homolog 2 has also been implicated as a controller of intestinal stem cell fate and is essential for the maintenance of adult intestinal stem cells (Tanaka, M., et al., (1997) Dev Biol. 190(10):55-65).
Specificity
This antibody recognizes Achaete Scute homolog 2 near the C-terminus.
Immunogen
His-tagged recombinant protein corresponding to human Achaete Scute homolog 2 near the C-terminus.
Application
Anti-Achaete Scute homolog 2 Antibody, clone 7E2 detects level of Achaete Scute homolog 2 & has been published & validated for use in WB, IC, ChIP.
Immunocytochemmistry Analysis: A previous lot was used by an independent laboratory in IC (Van de Flier, L.G., et al., 2009)
Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in ChIP (Hatzis, P., et al., 2008).
Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in ChIP (Hatzis, P., et al., 2008).
Quality
Evaluated by Western Blot in human placenta tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Achaete Scute homolog 2 in 10 µg of human placenta tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Achaete Scute homolog 2 in 10 µg of human placenta tissue lysate.
Target description
~20 kDa
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Genta Nagae et al.
Nature communications, 12(1), 5423-5423 (2021-09-21)
Hepatoblastoma (HB) is the most common pediatric liver malignancy; however, hereditary predisposition and acquired molecular aberrations related to HB clinicopathological diversity are not well understood. Here, we perform an integrative genomic profiling of 163 pediatric liver tumors (154 HBs and
Jarom Heijmans et al.
Cell reports, 3(4), 1128-1139 (2013-04-03)
Stem cells generate rapidly dividing transit-amplifying cells that have lost the capacity for self-renewal but cycle for a number of times until they exit the cell cycle and undergo terminal differentiation. We know very little of the type of signals
Jun Ye et al.
Cell cycle (Georgetown, Tex.), 17(8), 1014-1025 (2018-06-12)
The Wnt signaling pathway controls stem cell identity in the intestinal epithelium and cancer stem cells (CSCs). The transcription factor Ascl2 (Wnt target gene) is fate decider of intestinal cryptic stem cells and colon cancer stem cells. It is unclear
Yangyang Shang et al.
Oncotarget, 6(31), 30993-31006 (2015-08-27)
The role of Achaete scute-like 2 (Ascl2) in colorectal cancer (CRC) cell differentiation is unknown. LS174T, HT-29 and Caco-2 cells have high Ascl2 expression, while Lovo and SW480 cells have low Ascl2 expression. LS174T and HT-29 cells with Ascl2 knockdown
Zhongfeng Liu et al.
Aging and disease, 10(6), 1207-1220 (2019-12-04)
The molecular mediators underlying the effects of inflammation on neural stem cells (NSCs) are not fully characterized. In this study, we identified Ascl2 as a downstream basic helix-loop-helix (bHLH) transcription factor in NSCs following exposure to TNFα. Under normal conditions
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