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MAB2000

Sigma-Aldrich

Anti-Integrin β1 Antibody, clone HB1.1

clone HB1.1, Chemicon®, from mouse

Synonym(s):

CD29

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HB1.1, monoclonal

species reactivity

human, pig

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

suitability

not suitable for activity/function inhibition

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGB1(3688)

Specificity

Reacts with beta-1 subunit of VLA integrins

Application

Anti-Integrin β1 Antibody, clone HB1.1 detects level of Integrin β1 & has been published & validated for use in FC, IP, WB, IH.
Immunoprecipitation of VLA integrins from lysate of 10E6 cell equivalent requires 2 μg of MAB2000.

Flow cytometry: 10 μg/mL

Western blot

Immunohistochemistry in frozen tissue samples: 5 μg/mL

Induction of cell aggregation: 10 μg/mL. MAB2000 is a potent stimulator of cell aggregation tested in U937, Jurkat and human peripheral blood lymphocytes.

MAB2000 does not affect VLA integrin-mediated cell adhesion to matrix proteins.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Linkage

Replaces: 04-1109

Physical form

Format: Purified
Purified immunoglobulin from Protein A chromatography. Liquid in 0.02M PB pH 7.6, 0.25M NaCl with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C for up to 6 months after date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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