HKI2MAG-99K

MILLIPLEX^® Human Kidney Injury Magnetic Bead Panel 2 - Toxicity Multiplex Assay

The analytes available for this multiplex kit are: α-1-Microglobulin, Albumin, Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, and Osteopontin (OPN).

• Synonym(s): Human Kidney Toxicity Multiplex Assay, Luminex^® Human Kidney Toxicity Assay, Millipore Human Kidney Toxicity Panel
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 95-109%; sensitivity: 0.015-5.359 ng/mL; (MinDC = 2SD); standard curve range: 0.01-10 ng/mL; (EGF); standard curve range: 0.02-20 ng/mL; (Lipocalin-2/ NGAL); standard curve range: 0.20-200 ng/mL; (Cystatin C); standard curve range: 0.59-600 ng/mL; (Osteopontin (OPN)); standard curve range: 0.98-1,000 ng/mL; (α-1-Microglobulin); standard curve range: 3.91-4,000 ng/mL; (Albumin); standard curve range: 4.88-5,000 ng/mL; (Clusterin); inter-assay cv: <20%; intra-assay cv: <10%
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Maintenance of physiological balance in the body is attributed in part to normal kidney function. Altered kidney function due to injury, drug toxicity or kidney failure may be life threatening. Toxicity is the leading cause of drug failure, so research is expanding in search of more sensitive, rapid methods to determine organ-specific damage as quickly as possible. Traditionally, blood urea nitrogen (BUN) and serum creatinine tests have been used to determine drug-induced damage to the kidney. These tests only detect kidney damage after it begins and do not allow for the elucidation of where in the kidney the damage has occurred. Specificity and earlier detection of kidney injury is vital, and this area of research is expanding in search of more sensitive, rapid methods for determining the specific area of damage in kidney.

The MILLIPLEX^® portfolio provides valuable research assays to investigate multiple biomarkers of kidney injury in human urine samples using the Luminex^® xMAP^® instrument platform. The analytes available for this multiplex kit are: α-1-Microglobulin, Albumin, Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, and Osteopontin (OPN).

Panel Type: Toxicity

Specificity

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Application

• Analytes: α-1-Microglobulin, Albumin, Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, Osteopontin (OPN)
• Recommended Sample type: Urine
• Recommended Sample dilution: 1:100 in kit Assay Buffer. Customers need to determine the optimal dilution factor for their samples. Our recommendations are based on urine samples from normal subjects.
• Assay Run Time: Overnight
• Research Category: Toxicity

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Sensitivity: Please see kit protocol for individual assay sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H317,H318,H373,H411
• Precautionary Statements: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Target Organs: Respiratory Tract
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects