Skip to Content
Merck
All Photos(1)

Documents

GR15

Sigma-Aldrich

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

liquid, clone EGFR.1, Calbiochem®

Synonym(s):

Anti-Epidermal Growth Factor Receptor

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

EGFR.1, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

should not react with

rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

General description

Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, and IP.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
Recognizes the ~170 kDa EGF receptor protein in A431 cells. For neutralization studies Cat. No. GR15L is recommended.

Immunogen

Human
partially purified, human EGFR from A431 cells

Application


Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; use Cat. No. GR15L; see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM sodium phosphate, 0.2% gelatin, pH 7.5.

Analysis Note

Negative Control
HL-60 cells
Positive Control
A-431 cells

Other Notes

EGF-Receptor (Ab-5) binds to A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
Hunter T., 1984. Nature311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Nalo Hamilton et al.
BioMed research international, 2015, 925703-925703 (2015-04-16)
Triple-negative breast cancer (TNBC) occurs in 10-15% of patients yet accounts for almost half of all breast cancer deaths. TNBCs lack expression of estrogen and progesterone receptors and HER-2 overexpression and cannot be treated with current targeted therapies. TNBCs often
Jean-Philippe Grossier et al.
The EMBO journal, 33(1), 35-45 (2013-12-25)
Preferred sites of endocytosis have been observed in various cell types, but whether they occur randomly or are linked to cellular cues is debated. Here, we quantified the sites of endocytosis of transferrin (Tfn) and epidermal growth factor (EGF) in

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service