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CBL212

Sigma-Aldrich

Anti-Neurofilament 200 kDa Antibody, clone RT97

clone RT97, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

clone

RT97, monoclonal

species reactivity

rat, human, pig, avian, chicken, reptile

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Specificity

The clone RT97 recognizes the 200 kDa neurofilament polypeptide in a number of mammalian and avian species. Tumours specifically recognized include phaeochromocytoma, paraganglioma, ganglioneuroblastoma and other tumours of neuronal origin.

KNOWN SPECIES CROSS REACTIVITY: Recognizes rat, chicken, pig and reptile

Application

Anti-Neurofilament 200 kDa Antibody, clone RT97 detects level of Neurofilament 200 kDa & has been published & validated for use in IH, IH(P).
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers
Staining of both frozen and paraffin wax embedded tissue sections

Strong staining of neuronal processes in a variety of tissues

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified
The monoclonal is presented in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stephen M F Jamieson et al.
Molecular pain, 5, 66-66 (2009-11-20)
Oxaliplatin and related chemotherapeutic drugs cause painful chronic peripheral neuropathies in cancer patients. We investigated changes in neuronal size profiles and neurofilament immunoreactivity in L5 dorsal root ganglion (DRG) tissue of adult female Wistar rats after multiple-dose treatment with oxaliplatin
Signals for the initiation and termination of synthesis of the viral strand of bacteriophage f1.
Dotto, G P, et al.
Cold Spring Harbor Symposia on Quantitative Biology, 47 Pt 2, 717-722 (1983)
2-(Cyclohexylamino)-1-(4-cyclopentylpiperazin-1-yl)-2-methylpropan-1-one, a novel compound with neuroprotective and neurotrophic effects in vitro.
H Elbr?nd-Bek,A Wellejus,N M Kelly,M S Weidner,S H J?rgensen
Neurochemistry International null
Hisashi Hashimoto et al.
Neuro-degenerative diseases, 17(4-5), 181-198 (2017-05-11)
A novel ataxic mouse line was established from the offspring of a male mouse administered cyclophosphamide in a juvenile period. We have attempted to examine the phenotype and histopathological changes of affected mice. Furthermore, linkage analysis and sequencing of the
Adi Nitzan-Luques et al.
Pain, 152(10), 2413-2426 (2011-08-30)
Pain is normally mediated by nociceptive Aδ and C fibers, while Aβ fibers signal touch. However, after nerve injury, Aβ fibers may signal pain. Using a genetic model, we tested the hypothesis that phenotypic switching in neurotransmitters expressed by Aβ

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