AM80
Anti-PAR (Ab-1) Mouse mAb (10H)
liquid, ≥95% (SDS-PAGE), clone 10H
Synonym(s):
Anti-Poly (ADP-Ribose)
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
10H, monoclonal
Assay
≥95% (SDS-PAGE)
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, Drosophila, rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG3
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Protein A purified mouse monoclonal antibody. Recognizes poly-ADP-ribose.
Recognizes poly(ADP)-ribose (PAR) in etopside-treated HL-60 cells.
This Anti-PAR (Ab-1) Mouse mAb (10H) is validated for use in Immunoblotting, Immunocytochemistry for the detection of PAR (Ab-1).
Immunogen
Bovine
purified poly(ADP-ribose)
Application
Immunoblotting (2.5 µg/ml)
Immunocytochemistry (5-20 µg/ml)
Immunocytochemistry (5-20 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
Normal mouse IgG
Normal mouse IgG
Positive Control
Etoposide-treated HL-60 cells
Etoposide-treated HL-60 cells
Other Notes
Kawamitsu, H., et al. 1984. Biochemistry23, 3771.
PAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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J Wang et al.
Oncogene, 36(43), 5939-5947 (2017-06-27)
Understanding the mechanisms of platinum compound resistance, including cisplatin resistance, has important implications for improving cancer treatments. Previous studies identified a potential role for mitogen-activated protein kinase phosphatase-1 (MKP-1) in cisplatin resistance. This work focuses on the regulation of poly(ADP-ribose)
Li-Li Zhuang et al.
PloS one, 8(10), e77304-e77304 (2013-11-10)
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European journal of immunology, 40(8), 2174-2181 (2010-05-21)
We have previously shown that human Th17 lymphocytes are characterized by the selective expression of IL-23 receptor (IL-23R), CCR6, CD161, and the transcription factor retinoic acid-related orphan receptor C (RORC), and originate from a CD161(+)CD4(+) naïve T-cell precursor in response
Young-Ok Son et al.
Toxicology and applied pharmacology, 255(3), 287-296 (2011-07-20)
Cadmium is a toxic heavy metal which is environmentally and occupationally relevant. The mechanisms underlying cadmium-induced autophagy are not yet completely understood. The present study shows that cadmium induces autophagy, as demonstrated by the increase of LC3-II formation and the
Lingyu Zhang et al.
ACS omega, 7(3), 2571-2582 (2022-02-01)
Etoposide (VP-16) is used for the treatment of various cancers, including nasopharyngeal carcinoma (NPC); however, cancers develop resistance to this agent by promoting DNA repair. The DNA-PK (DNA-PKcs) catalytic subunit and poly(ADP-ribose) polymerase 1 (PARP1) mediate acquired resistance and poor
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