AB5407
Anti-Opsin Antibody, blue
Chemicon®, from rabbit
Synonym(s):
Anti-BCP, Anti-BOP
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
monkey, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... OPN1LW(5956)
General description
The full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.
Specificity
Recognizes Opsin, blue.
Immunogen
Epitope: blue
Recombinant human blue opsin.
Application
Immunohistochemistry: 1:200-1:300 on formalin-fixed, paraffin-embedded mouse retina tissue. Antigen retrieval method recommend is HIER with steam heat; other fixation and retrieval methods are untested.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Sensory & PNS
Sensory & PNS
This Anti-Opsin Antibody, blue is validated for use in IH(P) for the detection of Opsin.
Physical form
Format: Purified
Protein A purified
Purified immunoglobulinin PBS {0.02M phosphate, 0.25M NaCl, pH 7.6} with 0.1% sodium azide as a preservative
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment.
Analysis Note
Control
Retina
Retina
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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L P Morin et al.
Neuroscience, 199, 213-224 (2011-10-12)
Four studies were performed to further clarify the contribution of rod/cone and intrinsically photoreceptive retinal ganglion cells to measures of entrainment, dark preference, light-induced locomotor suppression and photosomnolence. Wild type (WT), retinally degenerate (rd/rd), and melanopsin-less (OPN4⁻/⁻) mouse strains were
Photoreceptor coupling mediated by connexin36 in the primate retina.
O'Brien, JJ; Chen, X; Macleish, PR; O'Brien, J; Massey, SC
The Journal of Neuroscience null
Prasanthi Namburi et al.
American journal of human genetics, 99(3), 777-784 (2016-09-03)
Inherited retinal diseases (IRDs) are a diverse group of genetically and clinically heterogeneous retinal abnormalities. The present study was designed to identify genetic defects in individuals with an uncommon combination of autosomal recessive progressive cone-rod degeneration accompanied by sensorineural hearing
Ji-Neng Lv et al.
Scientific reports, 7, 43062-43062 (2017-02-22)
Precursor messenger RNA (Pre-mRNA) splicing is an essential biological process in eukaryotic cells. Genetic mutations in many spliceosome genes confer human eye diseases. Mutations in the pre-mRNA splicing factor, RP9 (also known as PAP1), predispose autosomal dominant retinitis pigmentosa (adRP)
Histopathology and functional correlations in a patient with a mutation in RPE65, the gene for retinol isomerase.
Bonilha, VL; Rayborn, ME; Li, Y; Grossman, GH; Berson, EL; Hollyfield, JG
Investigative Ophthalmology & Visual Science null
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