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Sigma-Aldrich

β1-3,6-Galactosidase, Recombinant, E. coli

Synonym(s):

β1-3,6-Galactosidase, Recombinant, E. coli, β1-3,6-D-Galactoside Galactohydrolase

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About This Item

CAS Number:
Enzyme Commission number:
UNSPSC Code:
12352202
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

liquid

specific activity

≥30 units/mL
≥75 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

foreign activity

Proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

General description

Recombinant, β1-3,6-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,3- and β1,6-linked galactose from complex carbohydrates and glycoproteins. Not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
Recombinant, β1-3,6-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,3-and β1,6-linked galactose from complex carbohydrates and glycoproteins. Not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Other Notes

Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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