16-201
Protein G Agarose/Salmon Sperm DNA, 2.5 mL
for use in chromatin immunoprecipitations (ChIP assays)
Synonym(s):
ChIP agarose beads, ChIP agaraose G beads
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32
Recommended Products
Quality Level
form
liquid
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
suitability
suitable for immunoprecipitation
shipped in
wet ice
General description
Recombinant Protein G covalently bound to agarose by cyanogen bromide linkage. Sonicated salmon sperm DNA blocks non-specific DNA binding sites on protein G agarose when collecting immunocomplexes for chromatin immunoprecipitations (ChIP assays).
Recombinant protein G has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein G on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
Packaging
2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
Quality
Routinely evaluated in Chromatin Immunoprecipitation for use to pre-clear the supernatant from sodium butyrate treated HeLa cells and subsequent immunoprecipitation of DNA cross-linked to acetylated histones
Physical form
2.5 mL packed beads containing 1 mg sonicated salmon sperm DNA, 2.5 mg BSA and approximately 5 mg recombinant Protein G. Provided as a 50% gel slurry for a final volume of 5 mL per vial. Suspended in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, containing 0.05% sodium azide. Liquid suspension.
Storage and Stability
1 year at 4°C from date of shipment.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Steven McFarlane et al.
PLoS pathogens, 15(3), e1007667-e1007667 (2019-03-23)
Host innate immune defences play a critical role in restricting the intracellular propagation and pathogenesis of invading viral pathogens. Here we show that the histone H3.3 chaperone HIRA (histone cell cycle regulator) associates with promyelocytic leukaemia nuclear bodies (PML-NBs) to
Transcriptional regulation of CD4 gene expression by T cell factor-1/beta-catenin pathway.
Zhaofeng Huang, Huimin Xie, Vassilio Ioannidis, Werner Held, Hans Clevers et al.
Journal of immunology (Baltimore, Md. : 1950) (1950)
Innate mechanism of pollen- and cat dander-induced oxidative stress and DNA damage in the airways.
Koa Hosoki et al.
The Journal of allergy and clinical immunology, 140(5), 1436-1439 (2017-06-07)
Arthi Prabhakar et al.
PloS one, 14(3), e0213326-e0213326 (2019-03-27)
Optogenetics enables the selective activation of genetically-targeted neuronal populations using light-sensitive ion channels. Genetic strategies using Cre-dependent mouse strains, especially the Ai32 line expressing Channelrhodopsin (ChR2)-EYFP fusion protein, have been a popular means to drive opsin expression in a cell-type
Interaction between Hhex and SOX13 Modulates Wnt/TCF Activity.
Marfil V, Moya M, Pierreux CE, Castell JV, Lemaigre FP, Real FX, Bort R
The Journal of Biological Chemistry null
Related Content
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service