The Sigma-Aldrich Anti-α-Actin Mouse mAb (1A4) with the product number 113200 is the same "clone" as the Sigma-Aldrich Anti-Actin, smooth muscle Antibody, clone ASM-1/1A4 with the product number CBL171-I. Clone ASM-1, also known as anti-αsm-1 and clone 1A4. The Anti-Actin smooth muscle Antibody, clone ASM-1/1A4 (product number CBL171-I) shows reactivity in human, mouse, rat, chicken, and bovine species. Accordingly, the Anti-α-Actin Mouse mAb (1A4) with the product number 113200 should cross-react with bovine smooth muscle actin since it is the same clone as in CBL171-I.
113200
Anti-α-Actin Mouse mAb (1A4)
liquid, clone 1A4, Calbiochem®
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500 μL
€585.00
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500 μL
€585.00
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
1A4, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
baboon, rabbit, human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... ACTA1(58)
General description
Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Antibody Target Gene Symbol: ACTA2 Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN Entrez Gene Name: actin, alpha 2, smooth muscle, aorta Hu Entrez ID: 59 (Related Antibodies: 113200) Rat Entrez ID: 81633
This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.
Immunogen
Human Smooth Muscle
a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
Application
Frozen Sections (1:50-1:75)
Immunoblotting (see comments)
Paraffin Sections (1:50-1:75)
Immunoblotting (see comments)
Paraffin Sections (1:50-1:75)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
Analysis Note
Negative Control
Fibroblasts, striated muscle, or myocardium
Fibroblasts, striated muscle, or myocardium
Positive Control
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
Other Notes
Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Could you provide information on the reactivity of antibody 113200-500UL with bovine cells? The website does not mention reactivity for bovine species, and I would like to use it with bovine cells.
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