1.04022

Fast green FCF (C.I. 42053)

for microscopy Certistain^®

• Synonym(s): Fast green FCF (C.I. 42053), (C.I. 42053) for microbiology Certistain^®
• Empirical Formula (Hill Notation): C₃₇H₃₄N₂Na₂O₁₀S₃
• CAS Number: 2353-45-9
• Molecular Weight: 808.85
• MDL number: MFCD00013053
• UNSPSC Code: 41116130
• EC Index Number: 219-091-5

Properties

• Quality Level: 400
• form: solid
• potency: >2000 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥85% spectrophotometric assay
• pH: 2.7 (20 °C, 10 g/L in H₂O)
• mp: 290 °C (decomposition)
• solubility: 150 g/L
• bulk density: 350 kg/m³
• εmax: 1360-1610 at 622-626 nm in 50% ethanol at 0.003 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3-,40,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
• InChI key: XJBPDZVCYTYRIN-UHFFFAOYSA-L

Description

General description

The dry dye Fast Green FCF (C.I. 42053) for microscopy Certistain^®, is used for the preparation of a staining solution that can be used for modified connective tissue staining acc. to van Gieson in histological sections. Further it is applied in histology as counterstaining in e.g. trichrome staining according to Masson and additionally for histochemical staining, as well as in Cytology as counterstain instead of light green SF in Papanicolaou′s staining. As Certistain^® dye it is chemically quality-certified according to highest standards and strict specifications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 85 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 622 - 626 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 1360 - 1610
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H341
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(C): Not applicable